dnaE [2019-01-29 15:27:52]

lagging strand DNA polymerase III (alpha subunit), part of the [SW|replisome]

locus: BSU29230

pI: 6.02

mw: 125.14 kDa

protein length: 1115 aa

gene length: 3348 bp

function: [category|SW 3.1.1|DNA replication](lagging strand)

product: lagging strand DNA polymerase III (alpha subunit)

essential: yes

ec: 2.7.7.7

synonyms

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.1|DNA replication]
• [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]

Gene

Coordinates: 2,991,269 2,994,616

Phenotypes of a mutant

• essential [Pubmed|12682299]

The protein

Catalyzed reaction/ biological activity

• Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1) (according to Swiss-Prot)
• required for bacteriophage SPP1 replication [Pubmed|23268446]

Protein family

• DnaE subfamily (according to Swiss-Prot)

Structure

• [PDB|3E0D] (from ''Thermus aquaticus'', 39% identity) [Pubmed|18691598]

[SW|Localization]

• forms foci, the foci dissappear after the formation of DNA mismatches in a [protein|E2A8B04CD729332F9E6A13195181983F751F2F25|MutS]-dependent manner or after DNA replication arrest [Pubmed|21958350]
• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|65B5EA4DBDF5B260DED754B8A7B226AA88F17B55|dnaE]-[gene|45229A538D5124B60CE05DDC8699B9BC3D5AD9FF|ytsJ]
  description: [pubmed|22383849]

  regulatory mechanism

  • [protein|D267AF38B0CF107042326E9B8F3DD3C9C85840E4|LexA]: repression, [PubMed|14593098,16267290], in [regulon|D267AF38B0CF107042326E9B8F3DD3C9C85840E4|LexA regulon]

  regulation

  • induced by DNA damage ([protein|D267AF38B0CF107042326E9B8F3DD3C9C85840E4|LexA]) [http://www.ncbi.nlm.nih.gov/sites/entrez/14593098,16267290 PubMed]

References

Reviews

• 22933559,28073967

Original publications

• 11721055,14593098,16267290,20122408,23563155,23017159,21958350,23268446,24062730,24106089,18691598,28575448,28878042